Ko te Whakakotahitanga Peptide-Phase (SPPS): I roto i te SPPS, ka whakahiatohia nga peptides ki runga i te tautoko totoka, he kapia te tikanga.167393-62-6 Fmoc-L-Lys(Mtt)-OH ka whakamahia hei tetahi o nga poraka hanga mo te whakahiato peptide.Ko te roopu Fmoc (9-fluorenylmethoxycarbonyl) he roopu whakamarumaru e whakamahia nuitia ana i roto i te SPPS hei tiaki i te roopu amino o nga waikawa amino, hei aukati i nga tauhohenga taha e kore e hiahiatia i te wa o te hanganga here peptide.Ko te roopu Mtt (4-methyltrityl) tetahi atu roopu whakamarumaru, ka whakamahia hei tiaki i te roopu amino epsilon o nga toenga lysine.Ma tenei ka taea te whakakore me te hono i nga tauhohenga i te wa e whakaroa ana te peptide.
Te Tiaki Tiaki: 167393-62-6 Fmoc-L-Lys(Mtt)-OH ka taea te tango i raro i nga ahuatanga ngawari, te nuinga o te whakamahi i te waikawa penei i te waikawa trifluoroacetic (TFA).Ko tenei ka whakaatu i te roopu amino o te lysine, ka taea e ia te uru ki te hanganga here peptide me te waikawa amino e whai ake nei i roto i te raupapa.
Rautaki Tiaki Orthogonal: 167393-62-6 Fmoc-L-Lys(Mtt)-OH he maha nga wa e whakamahia ana me etahi atu waikawa amino e tiakina ana e te Fmoc me nga roopu tiaki rereke hei tuku i te whakahiato o nga peptides matatini me nga roopu mahi tiaki maha.Ko tenei rautaki ko te whakakore me te hono i nga waikawa amino motuhake i roto i te raupapa kua tohua hei hanga i te mekameka peptide e hiahiatia ana.
Whakarerekē Peptide: 167393-62-6 Fmoc-L-Lys(Mtt)-OH Ka taea hoki te mahi hei kakau mo etahi atu whakarereketanga.Whai muri i te whakahiato peptide, ka taea te tango i te roopu Mtt, ka waiho he roopu amino kore utu ki te toenga lysine mo te hono ki etahi atu ngota ngota, mo te mahi ano.